目的　通过nm2 3 H1的转化及导入BcaCD885细胞株 ,建立稳定、高效、低毒的转染方法 ,观察nm2 3 H1对BcaCD885细胞株侵袭转移能力的影响。方法　①利用基因转化技术 ,制备高纯度的nm2 3 H1真核表达质粒 ;②利用阳离子脂质体介导的转染技术 ,完成转染方法的建立 ;③利用免疫组化技术 ,检测转染前后的NDPKA的表达 ;④利用transwell小室和冲刷实验 ,观察转染前后细胞的侵袭、粘附、趋化运动能力的变化。结果　①使用重组的pCMV Neo Bam的真核表达载体 ,将nm2 3 H1转染口腔癌细胞 ,并获得稳定表达 ;②发现BcaCD885细胞株nm2 3 H1基因的转染前后表达水平有明显差异 ;③转染后的BcaCD885细胞侵袭、粘附、趋化运动能力均显著降低。结论　nm2 3 H1对BcaCD885细胞的侵袭转移能力具有显著抑制作用 Objective To establish a stable, efficient and low-toxic transfection method by transforming nm23 H1 into BcaCD885 cells and observe the effect of nm23 H1 on invasion and metastasis of BcaCD885 cells. Methods (1) The high purity nm2 3 H1 eukaryotic expression plasmid was prepared by gene transformation technology; (2) the establishment of transfection method by cationic liposome-mediated transfection; (3) the use of immunohistochemistry to detect before and after transfection Of NDPKA expression; ④ using transwell chambers and scouring experiments were observed before and after transfected cells invasion, adhesion, chemotactic motor ability changes. Results ① Using recombinant eukaryotic expression vector pCMV Neo Bam, we transfected nm2 3 H1 into oral cancer cells and obtained stable expression. ② The expression level of nm23 H1 gene in BcaCD885 cells was significantly different before and after transfection; Dyed BcaCD885 cells invasion, adhesion, chemotactic ability were significantly reduced. Conclusion nm23 H1 has a significant inhibitory effect on invasion and metastasis of BcaCD885 cells