论文部分内容阅读
目的:测定人工种植红景天不同药用部位花瓣、茎、根的含量。方法:取各1 g样品经过60%的乙醇溶解,超声波清洗器清洗50 min,过滤,旋蒸,加2 mL的甲醇,用微孔滤膜(0.45μm)过滤,采用CAPCELL PAK C18柱(2.0 mm×150mm,i.d.,3μm),柱温30℃,波长278 nm;样品温度10℃,进样体积10μL。流动相为甲醇-1%乙酸溶液45:55,流速为1 mL·min-1进行定量分析。结果:有效部位中的红景天苷含量从高到低依次为花瓣(35.00mg)、根(18.89mg)、茎(1.59 mg)。结论:建立了红景天中红景天苷含量的高效液相色谱方法,可适用于不同药材中红景天苷含量的测定。
Objective: To determine the content of petals, stems and roots in different medicinal parts of Artificial Rhodiola rosea. Methods: Each 1 g sample was dissolved in 60% ethanol, cleaned by ultrasonic cleaner for 50 min, filtered, vortexed and added with 2 mL of methanol, filtered through a microporous membrane (0.45 μm), and applied to a CAPCELL PAK C18 column mm × 150mm, id, 3μm). The column temperature was 30 ℃ and the wavelength was 278 nm. The sample temperature was 10 ℃ and the injection volume was 10μL. The mobile phase was methanol-1% acetic acid solution 45:55, the flow rate was 1 mL · min-1 for quantitative analysis. Results: The contents of salidroside in the effective parts from high to low were petal (35.00mg), root (18.89mg) and stem (1.59mg). Conclusion: The method of HPLC for the determination of salidroside in Rhodiola rosea L. is established, which is suitable for the determination of salidroside in different herbs.