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目的 对肺癌及相应的肺癌旁组织标本中蛋白激酶C(PKC)及其抑制物 (PKCI)的活性表达规律进行对照研究。方法 收集 2 6例肺癌及癌旁组织标本 ,通过检测同位素放射来测定标本胞浆和胞膜中PKC的比活性和PKCI对PKC活性抑制百分数。结果 2 6例肺癌组织细胞胞浆中PKC比活性显著高于癌旁组织 (P <0 .0 1) ;而非小细胞癌胸膜中PCK比活性明显低于癌旁组织 (P <0 .0 5 ) ,小细胞癌胞膜中PKC比活性与癌旁组织无明显差异 (P <0 ,0 5 )。另外 ,2 6例肺癌组织胞浆中PKCI的抑制活性明显低于癌旁组织 (P <0 .0 5 ) ,而胞膜中PKCI的抑制活性则与癌旁组织无显著差异 (P >0 .0 5 )。结论 肺癌组织中PKC被激活而活性增高 ,同时PKCI表达降低说明其对PKC的调控能力下降 ,以上表明PKC和PKCI可能在肺癌的发生及发展中起重要作用。
Objective To compare the expression of protein kinase C (PKC) and its inhibitor (PKCI) in lung cancer and its corresponding lung cancer tissues. Methods Twenty-six specimens of lung cancer and adjacent non-cancerous tissues were collected. The specific activity of PKC in cytoplasm and plasma membrane and the percent inhibition of PKC activity by PKCI were determined by detecting radioactive isotope emission. Results The PKC activity in cytoplasm of 26 lung cancer tissues was significantly higher than that in adjacent non-small cell lung cancer tissues (P <0.01), while the PCK specific activity in non-small cell lung cancer tissues was significantly lower than that in adjacent non-small cell lung cancer tissues (P <0. 5). The specific activity of PKC in small cell carcinoma cell membrane was not significantly different from that in paracancerous tissues (P <0, 0 5). In addition, the inhibitory activity of PKCI in cytoplasm of 26 lung cancer tissues was significantly lower than that in adjacent non-cancerous tissues (P <0.05), while the inhibitory activity of PKCI in the cytoplasm was not significantly different from that in adjacent non-cancerous tissues (P> 0.05). 0 5). Conclusions The activity of PKC is increased in lung cancer tissues. Meanwhile, the decrease of PKCI expression indicates that the regulatory capacity of PKC is decreased. These results suggest that PKC and PKCI may play an important role in the occurrence and development of lung cancer.