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用陆地棉中棉所7号(以下简称中_7)作母本与亚洲棉石系亚1号(以下简称石_1)作父本进行杂交,以中棉所7号自交作对照。将授粉后二天和三天的杂交和自交胚珠进行无菌离体培养,获得成熟的胚和幼苗。将培养70天左右的杂交和自交胚珠解剖观察胚胎发育情况及统计有胚的百分数,所得结果,种间杂交授粉(?)二天的有胚数为32.1％(每100个胚珠中有胚的胚珠数),三天的为42.1％;陆地棉自父授粉后二天的为22.7％,三天的为23.8％。从培养70天左右长成的杂交和自交“种子”中剖出的胚,转培于适宜生根的培养基,获得100多株幼苗。
Cotton with Gossypium hirsutum No. 7 (hereinafter referred to as _7) as the female parent and Asiatic cotton line Ya 1 (hereinafter referred to as stone _1) as the male parent to hybrid cotton self No. 7 as a control. Two and three days after pollination, the hybrid and self-propagated ovules were germ-free in vitro to obtain mature embryos and seedlings. The hybridization and self-fertilized ovules cultured for about 70 days were dissected to observe the embryonic development and the percentage of embryos was counted. The results showed that the number of embryos in two days after cross-pollination (?) Was 32.1% (embryos per 100 ovules Ovules), 42.1% in three days; Upland cotton 22.7% two days after pollination, 23.8% in three days. Embryos from the hybrid and selfed “seeds” grown for about 70 days were transferred to medium suitable for rooting to obtain more than 100 seedlings.