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目的研究卡巴胆碱对烫伤休克大鼠延迟复苏时补液量及脏器血流量的影响。方法将32只Wistar大鼠随机分为假烧伤组(A组)、标准组(B组)、对照组(C组)和卡巴胆碱组(D组),B、C、D组大鼠制备30%体表面积Ⅲ°烫伤烧伤模型。C组伤后半小时经口给予卡巴胆碱。烫伤后大鼠首先暴露颈静脉、动脉并切开插入无菌硅胶管,以微蠕动泵控制输入乳酸钠林格氏液,A组和D组按B组剂量或速度的1/2给予。A组只剪毛、行颈静脉、动脉切开插管,但不烫伤及补液。记录补液开始后0、1、2、4h各组的平均动脉压(MAP)、心率(HR)、血细胞比容(Hct);测定肝、肾、肠组织血流量。结果 D组2h、4h后可明显降低HR,提高MAP,改善烧伤休克大鼠早期各脏器的血流量。结论卡巴胆碱能改善烧伤休克大鼠早期脏器的血流量,减少补液量需求,有助于烧伤后休克及并发症的防治。
Objective To investigate the effect of carbachol on fluid volume and organ blood flow during delayed resuscitation in scalded shock rats. Methods Thirty-two Wistar rats were randomly divided into four groups: sham group (group A), standard group (group B), control group (group C) and carbachol group (group D) 30% body surface area Ⅲ ° scald burn model. Group C was given carbachol orally for half an hour after injury. Rats were scalded first expose the jugular vein, artery and incision into the sterile silicone tube to control the importation of lactate Ringer’s solution with a peristaltic pump, group A and D were given by group B dose or speed 1/2. A group only shearing, jugular vein, artery incision intubation, but not scalded and rehydration. The mean arterial pressure (MAP), heart rate (HR) and hematocrit (Hct) of each group were recorded at 0, 1, 2, 4 h after the start of rehydration. Blood flow of liver, kidney and intestine was measured. Results After 2 hours and 4 hours in group D, HR was significantly decreased, MAP was increased, and the blood flow in early organs of burn shock rats was improved. Conclusion Carbachol can improve the blood flow of early organs in burn shock rats and reduce the need of rehydration, which is helpful for the prevention and treatment of post-burn shock and complications.