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目的监测医院环境中曲霉菌含量、分布特点及分析其同源性。方法运用分离培养及DNA测序方法对医院不同病区、不同环境中真菌进行监测分析,并运用随机扩增多态性DNA(RAPD)方法分析其同源性。结果不同病区、不同环境曲霉菌污染程度不同,其中空气中以呼吸科、血液科最高,分别为325cfu/m3、250cfu/m3;空调出气口以肿瘤科、呼吸科、血液科较高,分别为0.176cfu/cm2、0.176cfu/cm2、0.118cfu/cm2;曲霉菌同源性分析(RAPD)结果示H4株(血液科空气)、H5株(呼吸科空气)、H6株(呼吸科空调出气口)之间,H7株(神经科空气)与H3株(神经科空调出气口)之间;Y1株(血液科空气)与Y4株(血液科空调出气口)之间,Y2株(消化科空气)与Y5株(呼吸科空气)之间的指纹图谱较一致。结论医院环境中曲霉菌污染较严重,且部分来源不同曲霉菌之间同源性较高。
Objective To monitor the content and distribution of Aspergillus in the hospital environment and analyze its homology. Methods The isolation and culture and DNA sequencing were used to monitor and analyze the fungi in different wards and different environments in the hospital. The homology was analyzed by random amplified polymorphic DNA (RAPD). Results There were different degrees of Aspergillus contamination in different wards and different environments. Among them, respiratory tract and hematology were the highest in the air, which were 325cfu / m3 and 250cfu / m3 respectively. Air-conditioned vents were higher in oncology, respiratory and hematology groups, 0.176cfu / cm2,0.176cfu / cm2,0.118cfu / cm2; Aspergillus oryzae homology analysis (RAPD) showed H4 strain (hematology air), H5 strain (Neurology air) and H3 (neurology air conditioning air outlet); between Y1 strain (hematology air) and Y4 strain (hematology air conditioning air outlet), Y2 strain Air) and Y5 strains (respiratory department air) fingerprint more consistent. Conclusion Aspergillus contamination in hospital environment is more serious, and homology is higher among some species of Aspergillus.