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目的 研究促胃液素拮抗剂生长抑素对促胃液素促大肠癌细胞生长作用的影响 ,为临床应用促胃液素拮抗剂治疗大肠癌提供实验依据。方法 应用3H TdR摄入法在体外检测大肠癌细胞株SW 480及 2 5例原代培养的人大肠癌细胞DNA的合成(cpm值 )情况。 结果 5肽促胃液素 (PG )组SW 480细胞及原代培养高、中、低分化腺癌细胞cpm值明显高于对照组 (P <0 .0 1) ,8肽生长抑素 (SMS)组cpm值明显低于对照组 (P <0 .0 1) ,PG +SMS组cpm值均明显低于PG组和对照组 (P <0 .0 1)。 结论 促胃液素拮抗剂生长抑素不仅对大肠癌细胞具有抑制作用 ,而且还具有抑制促胃液素对大肠癌细胞的促生长作用
Objective To study the effect of gastrin antagonist somatostatin on the growth of gastric carcinoma cells induced by gastrin, and provide experimental basis for the clinical application of gastrin antagonists in the treatment of colorectal cancer. Methods 3H TdR uptake method was used to detect the DNA synthesis (cpm value) of colorectal cancer cell line SW 480 and 25 primary cultured human colorectal cancer cells in vitro. Results The cpm value of 5-progesterone (PG) group was significantly higher than that of the control group (P < 0.01). The cpm value of the group was significantly lower than that of the control group (P <0.01). The cpm value of the PG + SMS group was significantly lower than that of the PG group and the control group (P <0. 01). Conclusion Gastrin antagonist somatostatin not only has inhibitory effect on colorectal cancer cells, but also has an inhibitory effect on the growth promoting effect of gastrin on colorectal cancer cells.