AIM:To investigate the immunotherapeutic potential ofvaccine consisting of dendritic cells (DCs) pulsed with totalRNA from MFC gastric cancer cells.METHODS:DCs were prepared from the spleens of strain615 mice by magnetic cell sorting (MACS).After culture for24 h,DCs were pulsed with total RNA from MFC gastric cancercells.Mice of one group were immunized with tumor RNApulsed DC (RNA/DC) at the dosage of 1×10~6 on d 14 and 7by s c inoculation before tumor implantation.Mice of anothergroup were immunized with unpulsed DC (UDC) at the samedosage on days as the RNA/DC group.The third group ofcontrol mice was untreated.On d 0,all the mice werechallenged with s c injections of 5×10~5 MFC gastric cancercells.After inoculation,the mice were monitored closely withrespect to tumor growth.Activities of NK cells in PBL andsplenocytes and CTL were tested.RESULTS:On d 21 after tumor cell inoculation,the miceof control group manifested the largest tumors with volumeat a mean of 2.6323±1.1435 cm~3,followed by the UDC andRNA/DC groups with mean volumes at 0.7536±0.3659 cm~3and 0.3688±0.6571 cm~3,respectively.The activities of NKcells in PBL and splenocytes in RNA/DC group were 66.2%and 65.4%,respectively,higher than that in the controlgroup.The tumor specific CTL activity in RNA/DC groupwas 49.5%,higher than that in the control group.CONCLUSION:The tumor vaccine with DCs pulsed withtotal RNA from gastric cancer cells possesses the ability tostimulate tumor specific CTL activity and to establish anti-tumor immunity when administered in vivo. AIM: To investigate the immunotherapeutic potential ofvaccine consisting of dendritic cells (DCs) pulsed with totalRNA from MFC gastric cancer cells. METHODS: DCs were prepared from the spleens of strain 615 of by magnetic cell sorting (MACS). After culture for 24 h, DCs were pulsed with total RNA from MFC gastric cancer cells. Mice of one group were immunized with tumor RNA pulsed DC (RNA / DC) at the dosage of 1 × 10 -6 on d 14 and 7 by sc inoculation before tumor implantation. Mice of another group were immunized with unpulsed DC (UDC) at the samedosage on days as the RNA / DC group. the third group of control mice was untreated. On d 0, all the mice were challenged with sc injections of 5 × 10 ~ 5 MFC gastric cancer cells. After inulation, the mice were monitored closely withrespect to tumor growth. Activities of NK cells in PBL andplenocytes and CTL were tested .RESULTS: On d 21 after tumor cell inoculation, the mice of control group manifested the largest tumors with volumeat a mean of 2.6323 ± 1.1435 cm -3 f ollowed by the UDC and RNA / DC groups with mean volumes at 0.7536 ± 0.3659 cm ~ 3 and 0.3688 ± 0.6571 cm ~ 3, respectively. These activities of NK cells in PBL and splenocytes in RNA / DC groups were 66.2% and 65.4%, respectively, respectively. than that in the control group. The tumor specific CTL activity in RNA / DC group was 49.5% higher than that in the control group. CONCLUSION: The tumor vaccine with DCs pulsed with total RNA from gastric cancer cells possesses the ability tostimulate tumor specific CTL activity and to establish anti-tumor immunity when administered in vivo.