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以细胞化学或者组织化学测定细胞内含物质的定性、定量,一般是在显微分光光度上测定相对含量。我们以XFG-01型显微分光光度计(国产)用单波长法,测定豚鼠曲细精管内的初级精母细胞及精子细胞核DNA的相对含量。这些比较规则的圆形或椭圆形结构,使用此法较易掌握,测得数值也较正确。现介绍如下: 一、材料和方法剖开豚鼠的睾丸被膜,用刀片将睾丸切成新鲜剖面並蘸一下生理盐水后涂片,经空气干燥,用Carnoy液固定,进行Feulgen法染色,用显微分光光度计测定初级精母细胞核和精子细胞核内DNA相对含量。各测定45个细胞。使用显微分光光度计的步骤: 1.选择与被测细线期的初级精母细胞及
The determination of the qualitative and quantitative content of cell contents by cytochemistry or histochemistry is generally determined by microspectrophotometry. We used the XFG-01 microspectrophotometer (domestic) to determine the relative content of primary spermatocytes and sperm cell nuclear DNA in the seminiferous tubules of guinea pigs using a single wavelength method. These relatively regular circular or elliptical structures are easier to grasp using this method and the measured values are more accurate. Are introduced as follows: First, the materials and methods cut open the guinea pig’s testicular capsule, cut the testes with a blade into a fresh section and wipe the saline after smear, air-dried, fixed with Carnoy solution, Feulgen staining, with microscopy A spectrophotometer was used to determine the relative amount of DNA in the primary spermatocyte nuclei and sperm nuclei. 45 cells were measured each. Steps for using a microspectrophotometer: 1. Select the primary spermatocytes at the fine line stage and