论文部分内容阅读
目的探讨低剂量硝酸镧对糖尿病大鼠视网膜B淋巴细胞瘤-2基因(Bcl-2)、B淋巴细胞瘤家族的前凋亡蛋白(Bax)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达水平的影响。方法将40只健康清洁级Wistar雄性大鼠随机分为3组,分别为对照组(10只)、糖尿病组(18只)和硝酸镧组(12只)。糖尿病组和硝酸镧组腹腔注射50 mg/kg链脲佐菌素制备糖尿病模型;硝酸镧组采用灌胃方式染毒0.2 mg/kg硝酸镧,对照组及糖尿病组均每日给予等剂量的生理盐水,每天1次,连续染毒1个月。采用免疫组织化学方法检测大鼠视网膜组织中Bcl-2、Bax和Caspase-3的蛋白表达水平。结果与对照组相比较,糖尿病组Bcl-2、Caspase-3的蛋白表达水平均显著降低、Bax的蛋白表达水平升高,差异有统计学意义(P<0.01);而硝酸镧组Bcl-2、Caspase-3的蛋白表达水平略降低、Bax的蛋白表达水平略有升高,差异无统计学意义(P>0.05);但与糖尿病组比较,硝酸镧组大鼠Bcl-2、Caspase-3的蛋白表达水平均显著升高,而Bax的蛋白表达水平显著降低,差异有统计学意义(P<0.01)。结论低剂量硝酸镧能够促进视网膜细胞Bcl-2、Caspase-3蛋白的表达及降低Bax蛋白的表达,从而起到保护视网膜的功能。
Objective To investigate the effect of low dosage of lanthanum nitrate on the expression of Bcl-2, Bax and caspase-3 in diabetic rat retinoblastoma (Bcl-2) Caspase-3) protein expression levels. Methods Forty healthy male Wistar rats were randomly divided into three groups: control group (n = 10), diabetes group (n = 18) and lanthanum nitrate group (n = 12). Diabetic group and lanthanum nitrate group were injected intraperitoneally with streptozotocin 50 mg / kg to prepare diabetic model. The lanthanum nitrate group was treated with 0.2 mg / kg lanthanum nitrate by intragastric administration, while the control group and diabetic group were given equal doses of physiology Saline, 1 day, continuous exposure to 1 month. Immunohistochemistry was used to detect the protein expression of Bcl-2, Bax and Caspase-3 in retina of rats. Results Compared with the control group, the protein expression of Bcl-2 and Caspase-3 in diabetic group was significantly decreased, while the protein expression of Bax was increased, the difference was statistically significant (P <0.01) (P> 0.05). However, compared with diabetic group, the expressions of Bcl-2, Caspase-3 and Caspase-3 in Lanthanide group were significantly lower than those in control group (P <0.01). However, the protein expression of Bax was significantly lower than that of Bax (P <0.01). Conclusion Low dose of Lanthanum nitrate can promote the expression of Bcl-2, Caspase-3 protein and decrease the expression of Bax in retinal cells, which can protect the retina.