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以野生大豆ZYD00006为供体亲本,黑龙江省主栽品种绥农14为轮回亲本,连续多年回交并自交,构建了高世代染色体片段代换系BC3F3代161个株行。该群体经多代回交的遗传背景相对一致,大大提高了QTL定位的准确度。结合单因素方差分析法和独立样本T检验法对群体进行QTL定位,共获得9个单株粒重的QTL,分布于7个连锁群。两种方法中均被检测到的有3个QTL,分别为QSW-J-1、QSW-J-2和QSW-G-1;QSW-G-1和QSW-G-2与已有研究结果相吻合;其余7个QTL为新发现QTL,可能是本材料特有位点;其中QSW-J-1的导入片段长度是7.0 c M,且加性效应值为-2.7 g,可作为继续研究的首选位点。
Using wild soybean ZYD00006 as donor’s parent, Suinong14, a main cultivar of Heilongjiang Province, was a reincarnated parent, which was backcrossed for many years and selfed to construct 161 lines of BC3F3 with high generation of chromosome segment substitution lines. The genetic background of this population after many generations of backcrossing is relatively consistent, greatly improving the accuracy of QTL mapping. One-way ANOVA and independent sample T-test were used to locate QTLs for the population. Nine QTLs for grain weight per plant were found, distributed in seven linkage groups. Three QTLs detected in the two methods were QSW-J-1, QSW-J-2 and QSW-G-1, respectively. QSW-G-1 and QSW- And the remaining seven QTLs were newly discovered QTLs, which may be characteristic of this material. The length of QSW-J-1 introduced fragment was 7.0 cM and the additive effect value was -2.7 g, which could be used for further study Preferred location.