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本文以陆地棉铃重近等基因系为材料 (大铃≥ 5.4g,小铃≤ 1 .7g) ,构建大小铃两个近等基因池。利用 AFLP标记技术对两基因池进行分析 ,64对引物组合共产生 3840条稳定、清晰扩增带。五条扩增带在两基因池间呈现多态性 ,其中四条差异带出现在大铃基因池 ,编号分别为 LB- 1、L B- 2、L B- 3和 LB- 4;一条出现在小铃基因池 ,编号为 SB- 1。模板浓度梯度分析表明 ,在一定范围内 ,酶切模板浓度对 AFL P结果影响较小。利用稍作修改的 CTAB法提取的棉花 DNA可用于AFLP分析。
In this study, two near-isogenic pools of boll-bell were constructed using the isogenic lines of cotton boll weevils as the material (boll ≥ 5.4 g, boll ≤ 1.7 g). Two gene pools were analyzed using the AFLP marker technique, and a total of 3840 primer pairs produced a total of 3840 stable and clearly amplified bands. The five bands showed polymorphism between the two gene pools, of which four bands were found in the boll gene pool numbered LB-1, L-2, L-3 and LB-4, respectively; one appeared in small Bell Gene Pool, number SB-1. The template concentration gradient analysis showed that within a certain range, the concentration of the digested template had little effect on the AFL P results. Cotton DNA extracted using the slightly modified CTAB method can be used for AFLP analysis.