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本研究以a—~(32)P—dCTP标记的HPV—16DNA为探针,应用限制性内切酶技术和Southern印迹杂交方法进一步分析了32例HPV—16DNA斑点杂交阳性的宫颈活检标本中HPV—16基因组的物理状态。结果表明:正常宫颈上皮和宫颈良性增殖性病变(宫颈湿疣)中,HPV—16DNA均以染色体外游离基因的形式存在;宫颈癌前病变(宫颈上皮内新生物)中,HPV—16DNA以游离和整合两种状态共存;宫颈恶性增殖性病变(宫颈癌)中,HPV—16DNA完全以整合的形式存在于宿主细胞基因组中。以上结果提示:宫颈上皮中HPV—16基因组的整合在宫颈癌的发生发展过程中可能起着重要作用。
In this study, a-32 P-dCTP-labeled HPV-16 DNA was used as probe to further analyze the HPV-16 DNA positive hybridization cervical biopsy specimens by restriction endonuclease and Southern blot hybridization -16 The physical state of the genome. The results showed that in normal cervical epithelium and cervical benign proliferative lesions (cervical condyloma), HPV-16DNA were present in the form of extrachromosomal free genes; HPV-16DNA in cervical precancerous lesions (cervical intraepithelial neoplasia) The integration of both states coexists; in cervical malignant proliferative lesions (cervical cancer), HPV-16 DNA is present in an integrated form in the host cell genome. The above results suggest that the integration of HPV-16 genome in cervical epithelium may play an important role in the development of cervical cancer.