The effect of rolling temperature on both two- and single-phase regions and annealing in a temperature range of 700-950°C on the mi-crostructure and mechanical properties of Ti-5Al-4V-2Fe-1Mo alloy was investigated. The results indicated that the best balance of strength and ductility is obtained by rolling in the two-phase region due to the globularization of the alpha phase and increase in its volume fraction. After rolling in the two-phase region, the ductility of the specimens annealed at 700 to 800°C increased because of the finer size and globularized alpha phase, while the reduction in strength was attributed to a decrease in the alpha phase volume fraction. However, at 950°C, the strength increased and ductility dropped by the formation of acicular alpha phase due to an increase in the phase boundary area. Annealing and aging after rolling in the beta-phase region increased the strength and decreased the ductility, which is attributed to the formation of a secondary alpha phase. A com-bination of favorable yield strength (1113 MPa) and elongation (13.3％) was obtained through rolling at 850°C followed by annealing at 750°C and aging at 570°C.