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[目的]探讨低强度微波辐射对γ射线致人早幼粒白血病HL-60细胞的凋亡率、线粒体膜电位(mitochondrial membrane potential,MMP)和胞内游离Ca2+浓度改变的影响。[方法]将人早幼粒白血病HL-60细胞随机分为对照组、单纯微波组、单纯γ射线组和联合组(微波+γ射线)。单纯微波组和联合组给予12μW/cm2微波照射,每天1 h,连续辐照3 d;第4天给予单纯γ射线组和联合组8 Gyγ射线照射。用钙离子依赖性磷脂结合蛋白-异硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)双标记法检测细胞凋亡率,流式细胞仪检测线粒体膜电位和胞内游离Ca2+浓度。[结果]与对照组相比,单纯γ射线组凋亡率明显增加(P<0.05);联合组凋亡率与单纯γ射线组相比显著降低(P<0.05)。与对照组相比,单纯γ射线组线粒体膜电位下降明显(P<0.05);联合组线粒体膜电位与单纯γ射线组相比显著升高(P<0.05)。与对照组相比,单纯γ射线组胞内游离Ca2+浓度明显升高(P<0.05);与单纯γ射线组相比,联合组胞内游离Ca2+浓度明显降低(P<0.05)。[结论]本实验条件下,8 Gyγ射线可以引起细胞凋亡率增加、线粒体膜电位下降和胞内游离Ca2+浓度增高,导致细胞损伤。预先900 MHz、12μW/cm2低强度微波照射能够显著减轻γ射线引起的细胞损伤,表现为细胞凋亡率减少、线粒体膜电位升高和细胞内游离Ca2+浓度降低。
[Objective] To investigate the effects of low-intensity microwave radiation on apoptosis, mitochondrial membrane potential (MMP) and intracellular free Ca2 + concentration in human promyelocytic leukemia HL-60 cells induced by γ-rays. [Methods] Human promyelocytic leukemia HL-60 cells were randomly divided into control group, simple microwave group, simple γ-ray group and combined group (microwave + γ-ray). The mice in the microwave group and the combination group were irradiated with microwave at 12μW / cm2 for 1 hour every day for 3 days. On the 4th day, the mice were irradiated with 8 Gy γ ray and the combination group. The rate of apoptosis was detected by double labeling with calcium-dependent phospholipid-binding protein-fluorescein isothiocyanate / propidium iodide (PI), the mitochondrial membrane potential and intracellular free Ca2 + concentration. [Results] Compared with the control group, the apoptotic rate in the group of γ-rays was significantly increased (P <0.05). The apoptosis rate of the combination group was significantly lower than that in the group of γ-rays alone (P <0.05). Compared with the control group, the mitochondrial membrane potential decreased significantly in the group of pure γ-rays (P <0.05). The mitochondrial membrane potential in the combination group was significantly higher than that in the group of pure γ-rays (P <0.05). Compared with the control group, the concentration of intracellular free Ca2 + in the γ-ray group was significantly increased (P <0.05). Compared with the pure γ-ray group, the intracellular free Ca2 + concentration in the combined group was significantly decreased (P <0.05). [Conclusion] Under the experimental conditions, 8 Gy γ ray can cause apoptosis rate increased, mitochondrial membrane potential decreased and intracellular free Ca2 + concentration increased, leading to cell damage. Pre-irradiation of 900 MHz and 12μW / cm2 under low-intensity microwave irradiation can significantly reduce the γ-ray-induced cell damage, which is manifested by the decrease of apoptosis rate, the increase of mitochondrial membrane potential and the decrease of intracellular free Ca2 + concentration.