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本文进行了天花粉蛋白对离体培养的葡萄胎细胞、早孕蜕膜细胞、人胎儿肾皮质部细胞、HeLa细胞和鼻咽癌上皮细胞的损伤作用的比较研究。为了探索天花粉蛋白选择损伤细胞是否存在专一结合细胞的可能性,又以不敏感的蜕膜细胞为材料,应用辣根过氧化物酶标记技术进行了天花粉蛋白与细胞结合和进入细胞内定位的实验。此外,并以肝癌细胞为材料研究了对细胞增殖率的影响。结果表明葡萄胎合体滋胚层细胞和胎盘合体滋胚层细胞一样都是最敏感的细胞,经1微克天花粉蛋白处理即出现形态上损伤,至50微克则大多数细胞呈现不可逆的退化损伤,而其它类型的细胞则表现出有相当大的抗性。过氧化物酶标记追踪天花粉蛋白在细胞内分布的结果表明天花粉蛋白与蜕膜细胞有一定的亲和力,提示天花粉蛋白似乎不存在严格的细胞结合专一性;而天花粉蛋白在细胞内的分布一般仅限于细胞质,这一现象似乎提示细胞质区可能就是天花粉蛋白直接作用的靶的范围。细胞增殖率的实验结果表明50微克以上的药物剂量对肝癌细胞增殖率有一定的抑制作用,剂量增加抑制作用也增加。但是,经过一定的修复期以后,细胞增殖率又可得到完全恢复,反映了对细胞生长抑制的可逆作用。上述实验结果进一步加强了天花粉蛋白对滋胚层细胞具有一定细胞损伤专一性的结论。本文并对细胞损伤专一性问题进行了讨论。
In this paper, the effects of trichosanthin on the injury of hydatidiform mole, early decidual cells, human fetal renal cortical cells, HeLa cells and nasopharyngeal epithelial cells in vitro were compared. In order to explore the possibility that trichosanthin can select specific cells for cell injury, and the unstimulated decidual cells as materials, the trichosanthin-binding protein and cell-binding and intracellular localization experiment. In addition, the effect of cell proliferation rate was studied on the basis of liver cancer cells. The results showed that the syncytiotrophoblast cells were the most sensitive cells in the syncytiotrophoblasts and morphologically damaged by 1 microgram trichosanthin protein. Most of the cells showed irreversible degeneration damage at 50 micrograms, while other types Of the cells showed considerable resistance. The results of the peroxidase-labeled trace of trichosanthin distribution in the cells showed that Trichosanthin had some affinity with the decidual cells, suggesting that Trichosanthin appears to lack strict cell-binding specificity. The distribution of trichosanthin in the cells is generally only Confined to the cytoplasm, this phenomenon seems to suggest that the cytoplasmic domain may be the direct target of trichosanthin. Experimental results show that the cell proliferation rate of more than 50 micrograms of drug dose on the proliferation of liver cancer cells have a certain degree of inhibition, increased dose inhibition also increased. However, after a certain period of repair, cell proliferation rate can be fully restored, reflecting the reversible effect on cell growth inhibition. The above experimental results further strengthen the Trichosanthin of adventitial cells have a certain degree of cell damage Conclusion. This article also discusses the issue of cellular damage specificity.