论文部分内容阅读
研究了 (1)在低温预处理过程中 ,不同相对湿度对大麦花药出愈率的影响 ;(2 )低温处理愈伤组织对植株分化的影响 ;(3)高频快速建立大麦胚性悬浮细胞系 .结果表明 ,低温预处理的较大相对湿度能明显促进花药的出愈率 ;低温处理愈伤组织能提高绿苗率 ;以 7周龄的花药胚性愈伤组织为起始材料高频快速建立了均质、分散性好的胚性悬浮细胞系 ,建立频率约为 2 5 %～ 5 0 % ;已 8～ 9周龄的花药胚性愈伤组织作为起始材料 ,难以建立胚性悬浮细胞系
(1) Effects of different relative humidity on the recovery rate of anther from barley during low temperature pretreatment; (2) Effect of callus on plant differentiation under low temperature; (3) Establishment of barley embryogenic suspension cells The results showed that the relative humidity of low temperature pretreatment can significantly promote the emergence rate of anther; low temperature treatment callus can improve the green shoot rate; embryo callus 7 weeks of age as the starting material high frequency Rapid establishment of a homogeneous and well-dispersed embryogenic suspension cell line with an established frequency of about 25% ~ 50%. Embryogenic callus from 8 to 9 weeks of age was used as starting material for embryogenic Suspension cell lines