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目的:观察并比较滴鼻和胃灌注尼尔雌醇对双侧卵巢切除术后所致大鼠鼻黏膜上皮细胞凋亡的保护作用及二者的差别。方法:将60只雌性大鼠随机分为:对照组(不切除卵巢)、A组(双侧卵巢切除)、B组(双侧卵巢切除加尼尔雌醇胃灌注)、C组(双侧卵巢切除加尼尔雌醇滴鼻),每组15只,用流式细胞仪分别检测各组动物术后15、30、60d鼻中隔黏膜阳性细胞百分率和荧光强度指数。结果:双侧卵巢切除后,大鼠鼻黏膜阳性细胞百分率增高;尼尔雌醇胃灌注后15d与对照组比较,差异有统计学意义(P<0.05),术后30d恢复正常;尼尔雌醇滴鼻后15、30d与对照组比较,差异有统计学意义(P<0.05),术后60d恢复正常。结论:尼尔雌醇胃灌注和滴鼻可通过减少凋亡细胞而保护大鼠鼻黏膜免受雌激素缺乏的损害,与胃灌注法相比,滴鼻法达到治疗效果所需时间较长。
OBJECTIVE: To observe and compare the protective effect of intranasal and gastric nallesolol on the apoptosis of nasal mucosal epithelial cells induced by bilateral ovariectomy in rats and their differences. Methods: Sixty female rats were randomly divided into control group (without ovariectomy), group A (bilateral ovariectomy), group B (bilateral ovariectomy with galectin gastric perfusion), group C Ovariectomized plus nilestiol nasal drops), 15 rats in each group. The percentage of nasal septum positive cells and the fluorescence intensity index of each group were detected by flow cytometry at 15, 30, 60 days after operation. Results: The percentages of nasal mucosa positive cells increased after bilateral ovariectomy. The levels of nasal mucosal positive cells increased after 15 days of gastric mucosal neal estriol instillation compared with the control group (P <0.05), and returned to normal after 30 days. The difference was statistically significant (P <0.05) between the 15th day and the 30th day after alcohol nasal drops and the control group, and returned to normal 60 days after the operation. Conclusion: Nilestiparular gastric perfusion and nasal drops can protect rat nasal mucosa from estrogen deficiency by reducing apoptotic cells. Compared with gastric perfusion, intranasal nasal instillation can prolong the therapeutic effect.