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本文用线栓法制备鼠右侧大脑中动脉闭塞模型，分离新生鼠前脑皮质、基底节神经元进行原代培养，以维持神经元存活的总数和长出神经突出超过１００μｍ的神经元数目分别来反映脑缺血灶周围组织提取液（ＩＢＴＥ）中神经营养因子（ＮＴＦｓ）和促进神经元突起生长的神经营养因子（ＮＰＦｓ）的存在及其活性。结果显示：缺血对侧ＢＴＥ和ＩＢＴＥ维持神经元存活的总数和长出神经突超过１００μｍ的神经元数目均较空白对照组明显增加（Ｐ＜０．０１）；ＩＥＴＥ的这种作用较缺血对侧脑组织提取液的作用更为显著；随着缺血后时间的延长，ＩＢＴＥ的神经营养活性有增加趋势。结果提示ＩＢＴＥ含有维持体外培养的神经元存活（ＮＴＦｓ）和促进神经突起生长（ＮＰＦｓ）的神经营养因子的存在。这对脑缺血后神经元的损伤修复可能起重要作用。
In this paper, the rat right middle cerebral artery occlusion model was prepared by the method of thread occlusion. The forebrain cortex and basal ganglion neurons of neonatal rats were isolated and cultured in primary culture to maintain the total number of neurons surviving and the number of neurons with neurites exceeding 100μm To reflect the presence and activity of neurotrophic factors (NTFs) and neurotrophic factors (NPFs) that promote the neurite outgrowth in the tissue surrounding the ischemic brain tissue extract (IBTE). The results showed that the total number of neurons surviving BTE and IBTE and the number of neurons growing neurites exceeding 100μm increased significantly (P <0.01) compared with that of the blank control group. This effect of IETE was more marked than that of ischemia Contralateral brain tissue extract more significant role; with the extension of time after ischemia, IBTE neurotrophic activity has an increasing trend. The results suggest that IBTE contains the presence of neurotrophic factors that maintain neuronal survival (NTFs) and promote neurite outgrowth (NPFs) in vitro. This may play an important role in the repair of neuronal damage after cerebral ischemia.