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目的 观察STAT3在小鼠胚胎神经管发生和神经上皮分化迁移阶段的时空表达规律,为探讨STAT3在胚胎神经系统发生和发育过程的作用及可能机制提供形态依据。方法 采用全胚胎原位杂交技术和免疫组织化学技术,观察STAT3在神经胚形成和神经上皮分化迁移阶段的表达。结果 ①E8.75d整个鼠胚几乎没有观察到STAT3mRNA的表达,在E9.5d ,STAT3mRNA在前脑泡急剧增多,到E10 .5d ,阳性信号出现在中脑泡,E11.5d时在后脑泡的一狭窄区域有STAT3mRNA的表达;②E13 .5d ,在侧脑室周围即未来大脑新皮层区域、间脑、发育中的视网膜、第四脑室周围的脑组织(未来的脑桥)、脊髓、三叉神经节和背根神经节C1均可观察到较强的STAT3蛋白阳性信号,E14 .5d和E15 .5d时,大脑皮层、间脑、视网膜、第四脑室周围以及发育中的脑桥和脊髓等部位观察到中等强度的STAT3蛋白表达。结论 STAT3可能参与了神经管的关闭和神经上皮的分化和迁移。
Objective To observe the temporal and spatial expression of STAT3 in neural tube formation and neural epithelial differentiation and migration of mouse embryos and to provide a morphological basis for exploring the role and possible mechanism of STAT3 in embryonic nervous system development and development. Methods Whole embryo in situ hybridization and immunohistochemistry were used to observe the expression of STAT3 in neurogenesis and neuroepithelial differentiation. Results (1) There was almost no STAT3 mRNA expression in E8.75d mouse embryos at all times. At E9.5d, the STAT3 mRNA increased dramatically in the anterior segment of the cerebral cortex. At E10.5d, the positive signal appeared in the midbrain and in E11.5d The expression of STAT3mRNA in the narrow area; ②E13.5d, in the lateral ventricle area that is the future brain cortex area, the diencephalon, the developing retina, the brain tissue around the fourth ventricle (the future pons), the spinal cord, the trigeminal ganglion and the dorsal The positive signal of STAT3 protein was observed in root ganglion C1. At E14.5d and E15.5d, moderate intensity was observed in the cerebral cortex, diencephalon, retina, periventricular fourth ventricle and developing pons and spinal cord. STAT3 protein expression. Conclusion STAT3 may be involved in the closure of the neural tube and the differentiation and migration of neural epithelium.