【摘 要】
：
采用外科手术方法从山羊四肢肌肉或背最长肌获取3mm×3mm肌肉束,置于1mg.mL-1 IV胶原酶中,室温消化30min,分散肌丝,在体视显微镜下分离肌丝;再用0.25%胰酶-EDTA于37℃下
【基金项目】
：
优质转基因肉羊新品种培育项目（2011ZX08008-003）, 国家高技术研究发展计划项目（863-2011AA100307-04）
论文部分内容阅读
采用外科手术方法从山羊四肢肌肉或背最长肌获取3mm×3mm肌肉束,置于1mg.mL-1 IV胶原酶中,室温消化30min,分散肌丝,在体视显微镜下分离肌丝;再用0.25%胰酶-EDTA于37℃下消化10min,以释放出骨骼肌卫星细胞（SSC）和其他细胞。连续5代在增殖培养体系（80%DMEM/F12＋10%胎牛血清＋10%马血清）中采用差速贴壁方法,收集接种后20min的贴壁细胞,对纯化的SSC采用免疫荧光方法检测SSC特异标志蛋白Pax-7,结果表明：（92.5±3.4）%细胞表达P
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