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为解决珍贵用材树种福建青冈种子易萌和人工育苗困难等问题,以成熟健康未发芽的福建青冈种子为试验材料,以不施外源激素为对照处理,分别利用5个浓度(0、10、20、30、40 mmol·L~(-1))的赤霉素和脱落酸对种子进行处理,并于激素处理后3、5、7、9和13 d测定种子胚根和子叶内抗氧化酶系统的活性。结果表明:2种外源激素处理对福建青冈种子萌发率的影响存在明显差异。随赤霉素浓度增加,种子萌发率呈逐渐增高的趋势,但处理间差异不显著(P>0.05)。低浓度(10~20 mmol·L~(-1))脱落酸处理可明显提高种子萌发率,高浓度(30~40 mmol·L~(-1))则呈抑制作用。随着外源激素处理时间的延长,胚根与子叶过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均呈先增强后不规则的变化趋势。在不同赤霉素浓度处理下,胚根过氧化氢酶(CAT)活性随时间的延长逐渐增强,子叶CAT活性呈倒“N”型变化趋势。施加40 mmol·L~(-1)脱落酸处理后,福建青冈种子胚根与子叶多酚氧化酶(PPO)活性明显低于对照处理,显著抑制种子萌发,可有效解决种子易萌性问题。
In order to solve the problems of the easy-to-use tree species of Fujian Cyclobalanopsis glauca and the difficulty of artificial seedling breeding, the mature, healthy and non-germinating seeds of Cyclobalanopsis glauca were used as experimental materials and the exogenous hormones were not applied as the control. Five concentrations (0, 10, 20, 30, 40 mmol·L -1) gibberellin and abscisic acid were used to treat the seeds, and the antioxidation of seed radicle and cotyledon was determined at 3, 5, 7, 9 and 13 days after hormone treatment Enzyme system activity. The results showed that there was a significant difference between the two exogenous hormones on the seed germination rate of Cyclobalanopsis chungii. With the increase of gibberellin concentration, the seed germination rate showed an increasing trend, but there was no significant difference between treatments (P> 0.05). Low concentrations (10-20 mmol·L -1) of abscisic acid treatment could significantly improve the seed germination rate, while high concentration (30-40 mmol·L -1) inhibited the seed germination. With the prolongation of exogenous hormone treatment, the activities of peroxidase (POD) and superoxide dismutase (SOD) in radicle and cotyledon increased firstly and then irregularly. Under different concentrations of GA3, the activities of catalase (CAT) in the radicles increased gradually with the prolongation of time, and CAT activity in the cotyledons showed a trend of “N”. After 40 mmol·L -1 ABA treatment, the activities of PPO in the radicle and the cotyledons of C. cuspidatum were significantly lower than those of the control, which significantly inhibited the seed germination and could effectively solve the problem of seed adornment.