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预防喉痉挛(LS)有效药物研究,需要建立能诱发连续性可重复的LS动物模型。现介绍Aviv等(1989)方法改进的技术细节并对8只犬实验效果作了评价。方法:8只犬下肢浅静脉插管注硫喷妥钠诱导麻醉,用氟烷、一氧化氮和氧混合维持麻醉深度。心电图和测股动脉血压监控。低位气管切开并持续正压麻醉下,用氢氧化氨诱发LS。用阴道镜撑开犬口腔,血管钳夹会厌向前暴露喉腔可观察声带内收运动。声门下放压力气囊测定声门下压力(IGP)。
Effective drug research for the prevention of laryngospasm (LS) requires the establishment of an animal model of LS that induces continuity and reproducibility. The technical details of the improved method by Aviv et al. (1989) are presented and the experimental results of eight dogs are evaluated. Methods: Eight dogs were anesthetized with thiopental sodium through the superficial vein of venous cannula. The depth of anesthesia was maintained by the mixture of halothane, nitric oxide and oxygen. ECG and measurement of arterial blood pressure monitoring. Lower tracheotomy and continuous positive pressure anesthesia induced with ammonia hydroxide LS. With a colposcope propped open the dog oral cavity, volar clamp epiglottis exposed throat can be observed adduction movement. Subglottic pressure balloon measured subglottic pressure (IGP).