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目的探讨三七总苷对二甲基亚硝胺(DMN)致肝纤维化模型大鼠的干预作用。方法 60只SD大鼠,随机选取10只作为正常组,其余50只以DMN复制中度肝纤维化模型,成功造模37只,选取8只作为模型组,剩余随机分为三七总苷组(8只)、干扰素组(8只)、三七总苷+干扰素组(7只)、生理盐水组(6只)。三七总苷组给予血栓通冻干粉1.5mg/100g、干扰素组给予注射重组人干扰素γ8.675mg/100g、三七总苷+干扰素组给予以上两种药物、生理盐水组给予等量生理盐水,以上均每日肌肉注射1次,连续给药8周后检测各组大鼠血清肝功能、透明质酸,结合常规病理HE、苦味酸-天狼星红染色,评价肝组织炎症及纤维化程度。结果正常组及各干预组与模型组血清总蛋白(TP)、白蛋白(Alb)、丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)和血清透明质酸比较差异均有统计学意义(P<0.05);各组分别与模型组大鼠肝纤维化分级半定量后分值比较差异均有统计学意义(P<0.05);三七总苷组、干扰素组、三七总苷+干扰素组SSS半定量计分分别与模型组比较,差异均有统计学意义(P<0.05)。结论三七总苷能够改善DMN所致肝纤维化大鼠肝功能,降低血清透明质酸含量,改善肝纤维化病理分级。
Objective To investigate the effects of Panax Notoginseng on rats with hepatic fibrosis induced by dimethylnitrosamine (DMN). Methods Totally 60 SD rats were randomly selected as the normal group and the other 50 were given DMN medium-density hepatic fibrosis model. 37 rabbits were randomly selected and 8 rabbits were selected as the model group. The remaining mice were randomly divided into three groups: 8), interferon group (8), Panax notoginseng + interferon group (7) and saline group (6). Panax ginseng group was given thrombolytic freeze-dried powder 1.5mg / 100g, interferon group given injection of recombinant human interferon γ8.675mg / 100g, Panax notoginseng + interferon group given the above two drugs, saline group given Volume of normal saline, the above intramuscular injection once a day, continuous administration of eight weeks after the detection of serum liver function in rats, hyaluronic acid, combined with routine pathology HE, picric acid - Sirius red staining, evaluation of liver inflammation and fiber Degree of. Results The levels of serum total protein (TP), albumin (Alb), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum hyaluronic acid in normal group, (P <0.05). There was significant difference in the scores of liver fibrosis grading between each group and the model group after semi-quantitative grading (P <0.05). The total saponins of Panax notoginseng group, interferon group (P <0.05). The semi-quantitative scores of SSS in Panax notoginseng and interferon group were respectively compared with those in model group (P <0.05). Conclusion Panax notoginseng can improve the hepatic function, reduce the serum hyaluronic acid content and the pathological grade of liver fibrosis in rats with hepatic fibrosis induced by DMN.