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目的 :分离培育阴道内嗜酸乳杆菌。方法 :通过筛选培养基组份及菌种防冻剂进行乳杆菌的培育和贮存。结果 :不同氮源、碳源及抑菌剂对嗜酸乳杆菌生长的影响及不同防冻剂对菌种活性的影响均有明显差异。结论 :选择适当的培养基和菌种保护剂利于嗜酸乳杆菌的大量生产及贮存。
Objective: To isolate and cultivate vaginal Lactobacillus acidophilus. Methods: Cultivation and storage of Lactobacillus by screening medium components and antimicrobial agents. Results: The effects of different nitrogen sources, carbon sources and bacteriostats on the growth of Lactobacillus acidophilus and the effects of different antifreeze agents on the activity of bacteria were significantly different. CONCLUSION: The selection of appropriate medium and strain protectants will facilitate the mass production and storage of Lactobacillus acidophilus.