用聚氰基丙烯酸正丁酯为载体材料，经乳化聚合法制备了不同配方的硫酸庆大霉素毫微球胶体溶液。对粒度及分布，药物的包封率和载药量，表面动电位，表面张力，浊度，比重，粘度，折射率，酸度及外观形态等物理化学特性进行了观察、测定和对比。实验结果有助于全面了解庆大霉素毫微球的物理化学特性，并为质量评价方法的建立提供了依据。 Using poly-n-butyl cyanoacrylate as carrier material, gentamycin sulfate nanocolloid colloidal solution with different formulations was prepared by emulsion polymerization. The physical and chemical properties such as particle size and distribution, drug entrapment efficiency and drug loading, surface electrokinetic potential, surface tension, turbidity, specific gravity, viscosity, refractive index, acidity and appearance morphology were observed, measured and compared. The experimental results help to fully understand the physicochemical properties of gentamicin nanospheres and provide a basis for the establishment of the quality evaluation method.