DNA linearization by confinement in nanochannels is an efficient approach for single-molecule DNA analysis.When confined in nanochannels, a coiled DNA molecule would naturally stretch out as a combined result of its elastic properties and the excluded volume effect depending on the dimensions of nanochannels.However, nanoconfinement based DNA manipulation still faces several bottlenecks.First of all, expensive lithography techniques are usually employed to produce devices with sub-100 nm nanochannels.