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A stable monolayer silver flm has been developed for localized surface plasmon resonance biosensor.The dextran-stabilized silver nanoparticles of about 30nm in diameter were prepared in an aqueous medium,from which the glucose served as a reducing agent for [Ag(NH3)2]+ and the dextran was the stabilizer of the generated AgNPs in case of the further Ag mirror reaction.UV-Vis spectroscopy and transmission electron microscopy(TEM)were carried out to investigate the AgNPs structures.And self-assembled layers of dextran-coated AgNPs were prepared on glass substrates primed with APTMS.The silver nanoparticle flm was characterized by Atomic force microscopy(AFM)while the stability of the flm was studied by UV-Vis spectra.Results depicted that the silver flm was stable,monolayer and homogeneously dispersed.Hence,we proposed its potential applications in different felds such as sensors.