Pollen tube guidance is a common process, analogous to axon guidance in animals, which involves cell-cell interactions and signaling between the male gametophyte (pollen) and the female gametophyte.The putative female cues to attract pollen tube have been shown to be Cys-rich defensin-like peptide (Okuda et al., Nature 2009).And we previously showed that the central cell plays an important role in the female control of pollen tube guidance as mutation in CENTRAL CELL GUIDANCE (CCG) abolishes this process (Chen et al., Plant Cell 2007).I will discuss progress on the role of CCG in pollen tube guidance.On the other hand, nothing is known on how pollen tube perceives and responds to the female cues, although a putative membrane K+ transporter was implicated in the process (Lu et al., Plant Cell 2011).In an effort to identify male mutations disrupting the perception of the female signals, we identified a pollen-specific mutation, pollen-defective in guidance1 (pod1) that blocks micropylar pollen tube guidance in Arabidopsis.POD1 encodes a novel ER luminal protein that is conserved in all eukaryotic organisms and is expressed in pollen, pollen tube and the synergid of the embryo sac.