The effects of thermoinducible strategies on the expression of recombinant E.coli producing human-li

来源 :The 5th Sino-US joint Conference of Chemical Engineering(第五届 | 被引量 : 0次 | 上传用户:nfast
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  In order to increase the production of HLC, the effects of different thermoinducible methods on the foreign gene expression of recombinant E.coli were investigated.Serials strategies with different induction temperature, pH, C/N ratio, and specific growth rate were adopted.The results indicate that induction temperature is dramatically effective on the expression of HLC.HLC may be degraded when the temperature was decreased below 37℃ after three hours induction at 42℃.
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