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The construction of organophosphate-degradation bacteria and microbial fertilizer were investigated in this report.1.Construction of phytate acid degradation bacteria;2.The research of microbial fertilizer;As high effective potassium and phosphate-dissolution silicate bacteria,the Culture Technology of KNP414 was studied.As to the results of single factor experiments,the optimum culture was 3﹪ starch,0.2﹪ yeast,0.1﹪ phosphate and 0.1﹪ MgSO<,4>.Orthogonal experiment indicated that the optimum combination was 1﹪ starch,0.2﹪ Yeast,0.1﹪ Na<,2>HP<,4> and 0.1﹪ MgSO<,4>.The conditions of culture were also investigate in flask.The optimum conditions in 500 ml flask were as follows:50ml medium,0.2﹪CaCO<,3>,5~10﹪ inoculum,250 rpm/min rotate speed.Under the optimal medium and fermentation conditions,KNP414 was cultured in 70L fermentor,and the product was certified to be regular by related department of government.3.The effect of microbio-fertilizer on the growth of plants.