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Gene delivery-based on non-viral DNA vehicle is an attractive approach than viral vector. Several synthetic non-viral DNA transfer systems have been developed to overcome physiological, as well as intracellular barriers. However, in vivo gene transfer efficacies with non-viral vectors have been rather low in comparison to viral vectors. The ideal non-viral vector for gene delivery should condense DNA into neutral complexes,avoid toxicity interactions with cells, mediate cell specific binding and avoid DNA degradation in acidic vesicles. Targeted delivery of non-virus vectors into cells is likely to improve the delivery efficiency. Galactosylated polyethyleneimine has been used to increase the transfection efficiency of DNA in hepatoma cells. And antibody-mediated targeting of particles and lipid-mediated targeting of DNA have been exploited to increase the transfection and gene delivery efficiency.Taken together, the complexes of Ag/Ab-PEI and DNA have the ability of self-assembly into Ag/Ab-PEI/DNA VLPs. This kind of Ag/Ab-PEI/DNA VLPs can be used to effectively transfect Huh7 cells, and induce stronger immune response than the mixture of antigen and DNA.