论文部分内容阅读
【目的】探讨1，25‐二羟胆骨化醇（活性维生素D ）对2型糖尿病大鼠肾损伤的影响。【方法】30只雄性Wistar大鼠随机分为普通饲料组即正常组（N组，10只）和高脂高糖饲料组（建模组，20只），2型糖尿病模型组建模成功后随机分为两组，每组10只，糖尿病组（M 组）经腹腔注射25．μg／（kg?d）花生油，D 组腹腔注射25．ug／（kg d．）溶于花生油中的1，25‐二羟胆骨化醇。8周后留取大鼠血标本，检测血糖、血肌酐及尿素氮；同时处死大鼠留取肾组织，Western Blot 检测肾组织中肿瘤坏死因子‐α（TNF‐α）、转化生长因子‐β1（TGF‐β1）并比较。【结果】M组、D组大鼠血糖、尿素氮、肌酐均显著高于N组（ P ＜00．5），但D组血糖、尿素氮及肌酐较M组明显降低（ P ＜00．5）；M组、D组大鼠肾组织中 TNF‐α、TGF‐β1水平显著高于 N组，而D组较M组明显下调（ P ＜00．5）。【结论】1，25‐二羟胆骨化醇可通过抑制糖尿病大鼠肾脏 TNF‐α、TGF‐β1的表达，对糖尿病大鼠肾脏损伤起保护作用。“,”Objective]To study the impact of 1 ,25 dihydroxycholecalciferol on kidney injury of type 2 diabetic rats .[Methods]30 male Wistar rats were randomly divided into normal diet group (N group ,10 rats) and high fat and high glucose diet group (model group ,20 rats) ,Type 2 diabetes model group were successfully set and randomly divided into two groups ,with 10 rats in each group ,diabetes mellitus group (M group) was treated by intraperitoneal injection of 2 5. g/(kg?d) of peanut oil ,and D group by intraperitoneal injection 2 5.μg/(kg?d) 1 ,25 dihydroxy‐cholecalciferol dissolved in peanut oil .After 8 weeks ,blood samples were collected to detect blood glucose ,creatinine and urea nitrogen;At the same time ,the rats were sacrificed and the kidney tissues were kept .Tumor necrosis factor alpha (TNF‐α) ,transforming growth factor beta 1 (TGF‐β1) were detected by Blot Western and compared with that in renal tissue[.Results]Blood glucose ,urea nitrogen and creatinine in M group and D group were significantly higher than those in N group ( P <0 0.5) ,but the blood glucose ,urea nitrogen and creatinine in D group were significantly lower than that in M group ( P <0 0.5);The levels of TNF‐α、TGF‐β1 in M group and D group were significantly higher than those in N group ,while the D group was significantly lower than that in M group ( P <0 0.5)[.Conclu‐sion]1 ,25 dihydroxycholecalciferol can inhibit expression of TNF‐α、TGF‐β1 of diabetic rats kidney and take protec‐tive effect on renal injury in rats with diabetes .