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采用响应面优化法对东北红豆杉多糖的活性炭脱色工艺进行了研究。以脱色率和多糖保留率为指标,在单因素试验基础上,利用Box-Benhnken中心组合设计和响应面分析法,系统考察了活性炭加入量、脱色温度、振荡频率和脱色时间等因素对脱色过程的影响。建立了东北红豆杉多糖脱色率的二次多项数学模型,通过对数学模型的方差分析可知在脱色过程中活性炭加入量是影响脱色率最大的因素。确定了利用活性炭对东北红豆杉多糖脱色的最佳工艺条件:活性炭加入量4.7%、脱色温度41℃、振荡频率152 r/min、脱色时间50 min。结果表明,在此工艺条件下,多糖脱色率和保留率分别为86.92%和89.58%。
Response surface optimization method was used to study the decolorization process of polysaccharides from Taxus cuspidata. On the basis of single factor experiment and Box-Benhnken central composite design and response surface analysis, the decolorization rate and polysaccharide retention rate were taken as indexes to investigate the effect of decolorization process such as the amount of activated carbon, decolorization temperature, oscillation frequency and decolorization time Impact. The second order multiple mathematical model of the decolorization rate of Taxus cuspidata was established. Through the analysis of variance of the mathematical model, it can be seen that the amount of activated carbon added during decoloration is the factor that affects the decolorization rate. The optimum conditions for the decolorization of Taxus cuspidata polysaccharide with activated carbon were determined: the amount of activated carbon added was 4.7%, the decolorization temperature was 41 ℃, the oscillation frequency was 152 r / min and the decolorization time was 50 min. The results showed that under these conditions, the decolorization rate and retention rate of polysaccharides were 86.92% and 89.58% respectively.