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目的建立同时测定舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H含量的液相色谱-质谱联用方法(UPLC-Q-TOF)。方法采用Waters Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7μm),流动相为乙腈-甲酸-水体系梯度洗脱,流速为0.4 mL.min-1,PDA检测190～400 nm扫描;进样量:2.0μL。结果尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H分别在10.31～206.24,3.28～65.52,6.36～127.24,8.24～164.88,10.18～200.52,3.14～62.72μg.mL-1内与峰面积呈良好线性关系;精密度、重复性和稳定性良好;加样回收率在99%～101%之间。结论所建立的UPLC-Q-TOF方法简便、准确、快速、重复性好,可用于舒脑欣滴丸中尿苷、鸟苷、阿魏酸、藁本内酯、洋川芎内酯I和H的含量测定,为该药的质量控制提供依据。
OBJECTIVE To establish a liquid chromatography-mass spectrometry (UPLC-Q-TOF) method for the simultaneous determination of uridine, guanosine, ferulic acid, ligustilide, and yangchongxiong lactone I and H in Shunaoxin pills. Methods Waters Acquity BEH C18 column (2.1 mm × 100 mm, 1.7 μm) was used. The mobile phase was acetonitrile-formic acid-water system. The flow rate was 0.4 mL.min-1. Sample size: 2.0 μL. Results Uridine, guanosine, ferulic acid, ligustilide, and yangchongxiong lactone I and H were 10.31 ~ 206.24,3.28 ~ 65.52,6.36 ~ 127.24,8.24 ~ 164.88,10.18 ~ 200.52,3.14 ~ 62.72μg respectively. mL-1 and the peak area showed a good linear relationship; precision, repeatability and good stability; sample recovery was between 99% to 101%. Conclusion The established UPLC-Q-TOF method is simple, accurate, rapid and reproducible, and can be used for the determination of uridine, guanosine, ferulic acid, ligustilide, Determination of the quality of the drug to provide the basis for quality control.