论文部分内容阅读
近日,国家知识产权局公开一件由中国农业科学院烟草研究所申请的发明专利:用于检测烟草对TMV抗性的N基因特异性引物对、检测方法及试剂盒。该发明提供一种用于检测烟草对TMV抗性的N基因特异性引物对、检测方法及试剂盒,特异性引物对包括上游引物和下游引物。检测方法为:在烟草N基因序列特异区域设计一对特异性引物;以待测烟草品种的DNA为模板,使用N基因特异性引物对进行PCR扩增;采用电泳检测PCR扩增
Recently, the State Intellectual Property Office opened an invention patent application by the Institute of Tobacco Research of Chinese Academy of Agricultural Sciences: a pair of N-specific primers for detecting tobacco resistance to TMV, a detection method and a kit. The invention provides an N gene-specific primer pair, a detection method and a kit for detecting tobacco resistance to TMV. The specific primer pair includes an upstream primer and a downstream primer. The method comprises the following steps of: designing a pair of specific primers in the specific region of the tobacco N gene sequence; using the DNA of the tested tobacco variety as a template and carrying out PCR amplification by using the N gene-specific primer pair; using PCR to detect the PCR amplification