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目的：研究钒酸根和钒氧离子分别作为磷酸根和二价金属离子的类似物作用于微管蛋白对其聚合过程的影响。方法：用浊度法和荧光光度法测定钒化合物对猪脑微管蛋白的作用。结果：钒酸根促进微管蛋白聚合，而钒氧离子则抑制其聚合，对微管蛋白荧光影响表明二者均使蛋白内源荧光淬灭，但钒酸根作用大于钒氧离子。结论：钒酸根作用类似于其它磷酸类似物如ＡｌＦ４和ＢｅＦ３－即促进微管蛋白的聚合，钒氧离子的作用类似于二价阳离子钙（Ⅱ），抑制微管蛋白聚合，虽二者均使微管蛋白内源荧光淬灭，但作用方式可能不同。
OBJECTIVE: To study the effect of vanadate and vanadyl ions acting on tubulin as an analogue of phosphate and divalent metal ions respectively. Methods: The effects of vanadium compounds on porcine brain tubulin were determined by turbidimetry and fluorimetry. Results: Vanadate promoted tubulin polymerization, while vanadyl inhibited its polymerization. The effect on tubulin fluorescence indicated that both quenched the endogenous fluorescence of the protein, but vanadate was more active than vanadyl. Conclusions: Vanadate acts similar to other phosphate analogs such as AlF4 and BeF3 - to promote the polymerization of tubulin. Vanadyl ions act similarly to divalent cationic calcium (II) and inhibit tubulin polymerization, both of which Tubulin endogenous fluorescence quenching, but the mode of action may be different.