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目的 探讨特异性反义寡脱氧核苷酸 (antisenseoligodeoxynucleotide ,ASODN)对人胃癌细胞端粒酶活性的影响。方法 应用脂质体包裹与端粒酶RNA模板区互补的ASODN ,处理人胃癌细胞系FGC85。用端粒重复扩增分析 (telomererepeatamplificationprotocol,TRAP) PCR ELISA检测法观察端粒酶活性的变化。结果 经脂质体包裹ASODN处理的胃癌细胞端粒酶活性受到明显抑制 ,并与ASODN的浓度和处理时间相关。结论 端粒酶RNA模板区ASODN能够抑制端粒酶的活性 ,可运用于实验性肿瘤基因治疗研究以及端粒酶与肿瘤关系的进一步探讨
Objective To investigate the effect of antisense oligodeoxynucleotide (ASODN) on telomerase activity in human gastric cancer cells. Methods Human gastric cancer cell line FGC85 was treated with liposomes encapsulated in ASODN complementary to the telomerase RNA template region. Telomerase activity was observed by telomere repeat amplification (TRAP) PCR ELISA. Results The telomerase activity of gastric cancer cells treated with liposome-encapsulated ASODN was significantly inhibited and was related to the concentration of ASODN and treatment time. Conclusion Telomerase RNA template region ASODN can inhibit the activity of telomerase, which can be applied to experimental tumor gene therapy research and further explore the relationship between telomerase and tumors.