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目的:研究双酚A(bisphenol A,BPA)对绒毛外滋养细胞(extravillous trophoblasts,EVT)迁移和侵袭能力的影响。方法:本研究共收集98例胎盘绒毛标本,采用随机分组的方式将EVT细胞分为对照组和不同浓度的BPA处理组(每组≥3例)。用免疫荧光法(immunofluorescence,IFC)对分离的对照组EVT细胞进行鉴定;BPA暴露48 h后,用流式细胞术(FCM)检测BPA暴露对EVT细胞活力、凋亡和增殖的影响;用Transwell法检测EVT细胞的迁移和侵袭能力。结果:(1)IFC显示EVT细胞中人类白细胞抗原G(human leukocyte antigen G,HLA-G)阳性细胞的比例即EVT细胞的纯度>92.7%;(2)流式细胞术和免疫印迹法显示非毒性剂量的BPA不影响EVT细胞的凋亡(F=1.808,P=0.233)、活力(F=0.624,P=0.609)和增殖能力(F=0.174,P=0.913;F=0.260,P=0.852);(3)Transwell显示EVT的迁移和侵袭能力随BPA浓度的增高而呈剂量依赖性增强(F=24.080,P=0.000;F=144.900,P=0.000)。结论:BPA以剂量依赖的方式增强EVT细胞的迁移和侵袭能力。
Objective: To investigate the effect of bisphenol A (BPA) on migration and invasion of extravillous trophoblasts (EVT). Methods: In this study, 98 cases of placental villus specimens were collected. EVT cells were randomly divided into control group and BPA-treated group (≥3 cases in each group). The isolated control group of EVT cells were identified by immunofluorescence (IFC). After BPA exposure for 48 h, the effects of BPA exposure on the viability, apoptosis and proliferation of EVT cells were detected by flow cytometry (FCM) Method to detect EVT cell migration and invasion ability. Results: (1) IFC showed that the percentage of human leukocyte antigen G (HLA-G) -positive cells in EVT cells was> 92.7% of that of EVT cells; (2) Flow cytometry and Western blot Toxic doses of BPA did not affect the apoptosis of EVT cells (F = 1.808, P = 0.233), viability (F = 0.624, P = 0.609) and proliferative capacity (F = 0.174, P = 0.913; ); (3) Transwell showed that the migration and invasion ability of EVT increased dose-dependently with the increase of BPA concentration (F = 24.080, P = 0.000; F = 144.900, P = 0.000). Conclusion: BPA enhances the migration and invasion ability of EVT cells in a dose-dependent manner.