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目的克隆胃癌肝转移的相关基因。方法将标本分成有肝转移和无肝转移的胃癌原发灶两组,每组9例。提取组织总RNA,反转录成cDNA,将RT-PCR的扩增产物在PAGE测序胶上电泳,经EB染色分离两组差异表达的基因片段,TA克隆差异片段后进行测序,用GenBank BLAST软件进行同源性分析。将其中获得的1个与人内源性逆转录病毒基因片段做为标志物,用RT-PCR检测其在肝转移胃癌、无肝转移胃癌组织中的表达。结果有肝转移与无肝转移胃癌组织之间存在明显的基因表达差异,差异明显的10个基因片段均呈高表达。与人内源性逆转录病毒同源的基因片段在有肝转移胃癌组织中有7例表达,在无肝转移胃癌组织中有2例表达(P=0．007)。结论肝转移以胃癌癌基因突变为主,人内源性逆转录病毒基因片段在有肝转移胃癌组织中的表达高于无肝转移胃癌组织。
Objective To clone the related gene of liver metastasis of gastric cancer. Methods The specimens were divided into two groups of primary gastric cancer with liver metastasis and no liver metastasis, 9 cases in each group. Total RNA was extracted from the tissue and reverse transcribed into cDNA. The amplified product of RT-PCR was electrophoresed on PAGE gel. The differentially expressed genes were separated by EB staining. The differentially expressed fragments were sequenced and cloned by GenBank BLAST software Homology analysis was performed. One human endogenous retrovirus gene fragment obtained therefrom was used as a marker, and its expression was detected by RT-PCR in liver metastatic gastric cancer and non-hepatic metastatic gastric cancer tissues. Results There was a significant difference in gene expression between liver metastases and non-hepatic metastatic gastric cancer tissues. The 10 genes with significant difference were all highly expressed. Gene fragments homologous to human endogenous retrovirus were expressed in 7 cases of liver metastatic gastric cancer tissues and in 2 cases of non-hepatic metastatic gastric cancer tissues (P = 0.007). Conclusions Liver metastasis is mainly caused by gastric cancer gene mutation. The expression of human endogenous retrovirus gene fragment in gastric cancer with liver metastasis is higher than that in liver cancer without metastasis.