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利用电泳分析法分析,红松17个种源的酯酶同工酶的变化状况,结果表明,酯酶由3个基因位点控制,不同种源间的EST—B_3,EST—B_1,EST—A_1的基因频率有显著的差异。EST—B位点在各种源内的平均基因多样度为56.25％,范围在42.46％—66.91％。它的基因变异,6.4％在种源间,93.6％在种源内。种源间的平均遗传距离为0.09,范围在0.00—0.34,且遗传距离与地理距离有相同的趋势。种源的基因频率与基因多样度有随纬度渐变的趋势,且这种变异趋势主要由1月份的均温作用的结果。
The results showed that the esterase was controlled by three loci. EST-B 3, EST-B 1 and EST- There is a significant difference in the gene frequency of A_1. The average genetic diversity of EST-B loci in various sources was 56.25%, ranging from 42.46% to 66.91%. Its genetic variation is 6.4% between provenances and 93.6% within provenances. The average genetic distance between provenances was 0.09, ranging from 0.00 to 0.34, and the genetic distance and geographical distance had the same trend. The genetic frequency and genetic diversity of provenances have a trend of gradual change with latitude, and this trend of variation mainly results from the average temperature in January.