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以具有极高抗水平的抗溴氰菊酯家蝇 Ｍｕｓｃａ ｄｏ ｍｅｓｔｉｃａ ｖｉｃｉｎａ Ｍａｃｑｕａｒｔ Ｄ Ｒ０ 品系为试虫， 模拟田间几种常见的用药方式 （ 混用、轮用、使用增效剂） ， 在室内进行平行汰选， 并以不用药和继续用原药汰选的为比较， 研究试虫在这几种用药方式下的敏感性变化及其变化机制。抗性家蝇用辛溴混剂、辛硫磷以及溴氰菊酯＋ Ｓ Ｖ１ 汰选后， 在 Ｆ１６ （ Ｆ１７） 代以前， 对溴氰菊酯及汰选药剂的抗性发展相对都比较缓慢； Ｆ１６ （ Ｆ１７） 代以后， 用溴氰菊酯＋ Ｓ Ｖ１ 汰选的家蝇对溴氰菊酯的敏感性迅速下降， 抗性发展很快。家蝇对溴氰菊酯的敏感性变化与药剂中溴氰菊酯的选择压有关。生化分析结果表明， 在不同用药方式汰选下， 家蝇体内酯酶、多功能氧化酶、谷胱甘肽 Ｓ转移酶、乙酰胆碱酯酶的酶活或特性发生了不同的变化。
In this study, we used the Musca do mestica vicina Macquart D R0 strain of deltamethrin-resistant housefly with very high resistance as a test insect to simulate several common field treatments (mixing, rotation and synergist) in the house To compare and choose to use drugs and continue to use the original drug for comparison, to study the worm insects in these modes of change in the sensitivity and its mechanism of change. Resistant houseflies developed relatively slower resistance to deltamethrin and eliminated drugs before the F16 (F17) generation after the selection of the synergists, phoxim, and deltamethrin + S V1 ; After F16 (F17) generation, the sensitivity of deltamethrin to housefly eliminated by deltamethrin + S V1 decreased rapidly and the resistance developed rapidly. The sensitivity of housefly to deltamethrin was related to the selective pressure of deltamethrin in the drug. The results of biochemical analysis showed that the changes of enzyme activity or characteristics of esterase, multifunctional oxidase, glutathione S-transferase, and acetylcholinesterase in Housefly differed with the selection of different treatment methods.