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目的探讨姜黄素对沙漠干热环境下大鼠生存率的影响。方法选择SPF级6~8周龄雄性SD大鼠40只,随机分为对照组、姜黄素低剂量预处理组、姜黄素中剂量预处理组、姜黄素高剂量预处理组、溶剂组。对照组,正常饲喂;低剂量组、中剂量组、高剂量组、溶剂组分别等体积灌胃连续7 d。置于西北地区特殊环境人工实验舱,设定沙漠干热环境气候模式(温度41℃,湿度10%,紫外线辐射度40W/m2)。观察各组大鼠机体的生命体征并每半小时监测核心体温变化,记录死亡时间。结果五组大鼠从被置于沙漠干热环境开始,0~30 min,姜黄素三个剂量组的核心体温与对照组、溶剂组比较无显著差异(P>0.05)。从60 min开始,60 min、90 min、120 min、150min、180 min、210 min、240 min7个时间点,低剂量组、中剂量组、高剂量组的核心体温低于对照组、溶剂组,差异有显著性(P<0.05)。从0~240 min各时间点低剂量组、中剂量组、高剂量组组间比较均无显著性差异(P>0.05)。对照组、姜黄素低、中、高剂量治疗组及溶剂组五组大鼠平均生存时间分别为(222.95±28.23)min、(231.35±40.96)min、(255.00±30.39)min、(263.80±56.71)min、(223.00±27.23)min。中、高剂量姜黄素预处理组大鼠的生存时间均明显长于低浓度预处理组、对照组和溶剂组(P<0.05),其中高剂量组效果显著(P<0.01)。结论姜黄素预处理能够提高沙漠干热环境大鼠的生存时间,提示姜黄素可能在提高沙漠干热环境大鼠耐热性方面发挥重要作用,姜黄素可能在沙漠干热环境中暑的防治方面具有潜在的临床应用价值。
Objective To investigate the effect of curcumin on the survival rate of rats under dry and hot desert environment. Methods Forty Sprague-Dawley (SD) male Sprague Dawley rats aged 6 to 8 weeks were selected and randomly divided into control group, low dose curcumin pretreatment group, curcumin middle dose pretreatment group, curcumin high dose pretreatment group and solvent group. Control group, normal feeding; low dose group, middle dose group, high dose group, solvent group were given the same volume for 7 days. It was placed in a special environmental artificial laboratory in the northwestern region to set the climate pattern of dry and hot desert environment (temperature 41 ℃, humidity 10% and ultraviolet radiation 40W / m2). The vital signs of the rats in each group were observed and the changes of the core temperature were monitored every half an hour. The death time was recorded. Results After being placed in the hot and dry desert environment for 5 to 5 minutes, there was no significant difference (P> 0.05) between the core body temperature of the curcumin three dose groups and the control group and the solvent group at 0-30 minutes. At 60 min, 90 min, 120 min, 150 min, 180 min, 210 min and 240 min, the core temperature of low dose group, medium dose group and high dose group were lower than those of control group, solvent group, The difference was significant (P <0.05). There was no significant difference between low dose group, medium dose group and high dose group at all time points from 0 to 240 min (P> 0.05). The mean survival time in the control group, curcumin low, middle and high dose treatment group and solvent group were (222.95 ± 28.23) min, (231.35 ± 40.96) min, (255.00 ± 30.39) min, (263.80 ± 56.71) ) min, (223.00 ± 27.23) min. The survival time of middle and high dose curcumin pretreatment group was significantly longer than that of low concentration pretreatment group, control group and solvent group (P <0.05), and the effect of high dose group was significant (P <0.01). Conclusion Curcumin pretreatment can improve the survival time of rats in dry and hot desert environment, suggesting that curcumin may play an important role in improving the heat tolerance of rats in dry and hot desert environment. Curcumin may have the effect of prevention and treatment of heat stroke in desert dry and hot environment Potential clinical application value.