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目的 观察可溶性晚期糖基化终末产物受体(sRAGE)对动脉粥样硬化(AS)家兔主动脉组织细胞间粘附分子-1(ICAM-1)及核转录因子KB(NF-kB)表达的影响,探讨sRAGE抗动脉粥样硬化的可能机制.方法 24只家兔随机分为对照组、动脉粥样硬化模型组、sRAGE组及辛伐他汀组,每组各6只.对照组饲喂普通饲料,其余三组均饲喂高脂饲料10周,第6周起sRAGE组开始腹腔注射sRAGE,辛伐他汀组添加药物饲料.10周后检测家兔血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平;采用免疫组织化学法检测主动脉组织ICAM-1和NF-kB表达水平;蛋白质印迹法检测主动脉组织ICAM-1蛋白表达水平.结果 与对照组比较,AS模型组血清TC、TG、LDL-C水平明显升高,HDL-C水平明显降低(P＜0.05);SRAGE组血清总胆固醇、甘油三酯、LDL-C水平均低于AS模型组(P＜0.05),血清HDL-C水平高于AS模型组(P＜0.05),而与辛伐他汀纽比较差异无统计学意义(P＞0.05).与对照组相比,AS模型组主动脉组织中ICAM-1、NF-kB的表达水平明显升高(P＜0.05);与AS模型组比较,sRAGE组主动脉组织中ICAM-1、NF-kB的表达水平显著下降(P ＜0.05),而与辛伐他汀组比较差异无统计学意义(P ＞0.05).结论sRAGE可能通过抑制NF-kB及ICAM-1的表达,从而发挥抗动脉粥样硬化作用.“,”Objective To investigate the effects of soluble receptor for advanced glycation end products (sRAGE) on the expression of intercellular adhesion molecule- 1 and nuclear factor- kappa B in aortic tissues of atherosclerotic rabbits. Methods Twenty- four rabbits were randomly divided into four groups, including the control group, the atherosclerosis model group, the sRAGE group and the simvastatin group, each group had 6 rabbits. The control group were fed with normal diet, and the other three groups were fed with high fat diet for 10 weeks. The sRAGE group accepted intraperitoneal injection of sRAGE at sixth weeks, and the simvastatin group were supplemented with medicated feed.10 weeks later, serum total cholesterol, triglyceride, HDL and LDL levels were tested; Immunohistochemistry was employed to detect the expression of ICAM-1 and NF- κB in aortic tissues; Western blot was used to detect ICAM- 1 protein expression in aortic tissues. Results Compared with the control group, the levels of TC, TG and LDL- C in the AS model group were significantly increased and the levels of HDL- C were significantly decreased (P ＜0.05). Compared with the atherosclerosis model group, total cholesterol, triglyceride, low density lipoprotein levels were significantly lower in sRAGE group (P ＜ 0.05), and high density lipoprotein levels were significantly higher in sRAGE group (P ＜0.05), and no significant difference was observed between the atherosclerosis model group and simvastatin group (P ＞0.05). Compared with the control group, the expression of ICAM- 1 and NF- kB in the aortic tissue of the AS model group was significantly higher (P＜0.05). Compared with the atherosclerosis model group, the expression of ICAM-1 and NF- kB in the aortic tissues of sRAGE group were significantly decreased (P ＜0.05), and no significant difference was observed between the atherosclerosis model group and simvastatin group (P ＞0.05), Conclusion sRAGE may play an anti- atherosclerotic effect by inhibiting the expression of ICAM-1 and NF- kB.