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目的 观察穿心莲有效成分———API (API)对溶栓后再闭塞的预防作用 ,并探讨其可能机制。方法 用血管外电刺激方法复制冠脉血栓模型 ,溶栓后 14只犬被随机分为API治疗组 (n =6 )和对照组(n =8) ,治疗 4周。溶栓 4h、 3d、 30d测定血浆凝血恶烷B2 (TXB2 ) 6 酮前列腺素F ,组织型纤溶酶原激活剂 ,纤溶酶原激活剂抑制剂水平 ,并进行组织病理学检查。结果 治疗组再闭塞率为 16 7% ,对照组为87 5 % ,P <0 0 1。血浆TXB2 显著低于对照组 (P <0 0 1,P <0 0 1,P <0 0 5 ) ,30d对照组血浆血小板颗粒膜蛋白 (GMP - 14 0 )为基础水平的 3倍 ,而治疗组接近基础值 (与对照组比较P <0 0 0 1)。结论 穿心莲有效成分API有预防溶栓后再闭塞作用 ,机制与其抗血小板活化 ,抑制TXA2 产生等有关。
Objective To observe the preventive effect of API (API) on thrombolytic reocclusion and explore its possible mechanism. Methods Extracranial electrical stimulation was used to replicate the coronary thrombosis model. After thrombolysis, 14 dogs were randomly divided into API treatment group (n=6) and control group (n=8) for 4 weeks. The levels of plasma thromboxane B2 (TXB2), 6-ketoprostaglandin F, tissue plasminogen activator, and plasminogen activator inhibitor were measured at 4h, 3d, and 30d after thrombolysis, and histopathological examinations were performed. Results The reocclusion rate was 167% in the treatment group and 87.5% in the control group, P<0.01. Plasma TXB2 was significantly lower than that in the control group (P<0.01, P<0.01, P<0.05), and plasma platelet granule membrane protein (GMP-14) was 3 times higher than the baseline level in the 30-day control group. The group was close to the basal value (P < 0 0 0 1 compared with the control group). Conclusion The active ingredient API of Andrographis paniculata has the effect of preventing thrombolysis and blocking after thrombolytic therapy. Its mechanism is related to its anti-platelet activation and inhibition of TXA2 production.