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脉冲电场(pulsed electric field,PEF)对作物抗旱性的影响是电场生物学效应研究的重要课题,作物叶片延迟荧光动力学参数可以从不同角度灵敏地反映叶片细胞光合系统发生的变化。为了从活体细胞角度揭示脉冲电场对作物幼苗抗旱性的影响及其机理,使用频率为1Hz、场强为200kV·m~(-1)、脉宽为80ms的PEF处理萌发玉米种子,再采用渗透势为-0.1MPa的PEG-6000溶液形成干旱胁迫,研究了玉米幼苗生长过程中叶片干质量和LED诱导的叶片延迟荧光动力学参数的变化。结果发现,在-0.1 MPa的PEG-6000溶液形成的干旱胁迫下,玉米幼苗叶片干质量逐渐增加,经过PEF处理的玉米幼苗叶片干质量大于对照,相对增长率在5.8%~18.7%之间(p<0.05)。叶片延迟荧光动力学分析显示,干旱胁迫下玉米幼苗叶片延迟荧光动力学参数初始光子数I0、相干时间τ、衰减参数β和延迟荧光积分强度I(T)都发生了波动性的变化,这些变化是叶片细胞对干旱胁迫的适应性反应,PEF处理使玉米幼苗叶片延迟荧光各动力学参数和延迟荧光积分强度均明显大于对照组,表明PEF处理使玉米幼苗叶片细胞的光合潜力、组织序性和功能分子之间的相互作用都有所加强,叶片综合光合能力提高了。研究结果为阐明PEF对作物幼苗抗旱性的影响及其机理提供参考。
The effect of pulsed electric field (PEF) on crop drought resistance is an important issue in the study of electric field biological effects. Delayed fluorescence kinetic parameters of crop leaves can sensitively reflect the changes of leaf photosynthetic system from different angles. In order to reveal the effect and mechanism of pulse electric field on the drought resistance of crop seedlings from the perspective of living cells, the germination of maize seeds was treated with PEF at a frequency of 1 Hz, a field strength of 200 kV · m -1 and a pulse width of 80 ms, The PEG-6000 solution with a potential of -0.1MPa formed a drought stress, and the leaf dry weight and LED-induced delayed fluorescence kinetics parameters in the growth of maize seedlings were studied. The results showed that the dry weight of maize seedling leaves increased gradually under the drought stress of -0.1 MPa PEG-6000 solution. The dry weight of maize seedling leaves after PEF treatment was higher than that of the control, with the relative growth rate of 5.8% -18.7% p <0.05). Delayed fluorescence kinetics analysis of leaves showed that the fluctuation of initial fluorescence photon number I0, coherence time τ, decay parameter β and delayed fluorescence integral intensity I (T) of maize seedling leaves under drought stress all fluctuated. These changes Is the adaptive response of leaf cells to drought stress. The delayed fluorescence kinetics parameters and the delayed fluorescence integral intensities of leaves of maize seedlings were significantly greater than that of the control group by PEF treatment, indicating that PEF treatment can make the photosynthetic potential, Interaction between functional molecules have been strengthened, the comprehensive ability of photosynthesis leaves increased. The results provide a reference for elucidating the effects of PEF on the drought resistance of crop seedlings and their mechanism.