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A coumarinocoumarin-based fluorescent probe, JCCA, was developed for the detection of N2H4. JCCA exhibited a fast t-on fluorescence enhancement in response to N2H4 with good selectivity, sensitivity and a detection limit of 7.4 nmol/L. Significantly, JCCA displayed a good capability for visualizing N2H4 in living cells and zebra fish.