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[目的]研究66例嗜铬细胞瘤的临床病理、免疫组化及超微结构。[方法]对石蜡切片用ABC法避免疫级化染色，并对增殖细胞核抗原植效（PCNALI）进行计算。对新鲜瘤组织采用3％戊二醛和1％酸固定，超薄切片用醋酸轮和柠檬酸铅双重染色透射电镜观察。[结果]免疫级化染色瘤细胞表达Chromogranin阳性（20／23），NSE阳性（19／23）和synaptophysin阳性（17／23）。8例作了电镜检查在瘤细胞中可见神经内分泌颗粒。［结论］提示PCNALI对恶性嗜铬细胞瘤的诊断具有重要价值。电镜检查对于嗜锭细胞瘤与肾上腺皮质肿瘤的鉴别具有重要意义。
[Objective] To study the clinical pathology, immunohistochemistry and ultrastructure of 66 cases of pheochromocytoma. [Methods] Paraffin sections were evaded with immunohistochemical staining by ABC method, and PCNALI was calculated. Fresh tumor tissue was fixed with 3% glutaraldehyde and 1% acid, and ultrathin sections were observed with a double-staining transmission electron microscope with acetate wheel and citrate. [Results] The immunohistochemically stained tumor cells expressed Chromogranin positive (20/23), NSE positive (19/23) and synaptophysin positive (17/23). In 8 cases, electron microscopy examination revealed neuroendocrine particles in the tumor cells. [Conclusion] It is suggested that PCNALI has important value in the diagnosis of malignant pheochromocytoma. Electron microscopy has important implications for the identification of eutopic and adrenocortical tumors.