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目的:探讨胆管癌FIG-R0S融合基因的致瘤性,揭示其与胆管癌发生、发展的关系。方法:构建表达FIGROS融合基因的细胞稳转株,分别在体内、外研究其致瘤能力。结果:体外试验中,表达FIG-ROS的3T3及BaF3细胞稳转株在无IL-3的培养基中均保持生长。体内试验中,裸鼠皮下注射表达FIG-ROS的3T3细胞稳转株见肿瘤形成。结论:胆管癌中发现的ROS融合基因在体内、外均具有致瘤性,可能与胆管癌发生、发展相关。
OBJECTIVE: To investigate the tumorigenicity of the FIG-R0S fusion gene in cholangiocarcinoma and its relationship with the occurrence and development of cholangiocarcinoma. Methods: Stable cells expressing FIGROS fusion gene were constructed and their oncogenicity was studied in vitro and in vivo respectively. Results: In vitro experiments, 3T3 and BaF3 cells expressing FIG-ROS maintained growth in IL-3 free medium. In vivo, tumor formation was seen in nude mice injected subcutaneously with 3T3 cells stably expressing FIG-ROS. Conclusion: The ROS fusion genes found in cholangiocarcinoma both have tumorigenicity in vitro and in vivo, which may be related to the occurrence and development of cholangiocarcinoma.