甲氨蝶呤已成功应用于临床多年，关于其毒性及耐药性机制的研究也较深入。该文主要从甲氨蝶呤多聚谷氨酸化(PG化)与相关酶的关系这一角度阐述其在白血病治疗中的研究进展及临床应用。 Methotrexate has been successfully used in clinical for many years, and its mechanism of toxicity and drug resistance has also been studied in depth. This article mainly discusses the research progress and clinical application of methotrexate polyglutamination (PG) and related enzymes in the treatment of leukemia.